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Multifocal plane microscopy (MUM) or Multiplane microscopy or Biplane microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.〔 〕〔 〕〔 〕〔 〕 In this methodology, the light collected from the sample by an infinity-corrected objective lens is split into two paths.〔 〕 In each path the split light is focused onto a detector which is placed at a specific calibrated distance from the tube lens. In this way, each detector images a distinct plane within the sample. The first developed MUM setup was capable of imaging two distinct planes within the sample. However, the setup can be modified to image more than two planes by further splitting the light in each light path and focusing it onto detectors placed at specific calibrated distances. Another technique called multifocus microscopy (MFM) uses diffractive Fourier optics to image up to 25 focal planes. Presently, MUM setups are implemented that can image up to four distinct planes.〔 〕〔 〕 ==Introduction== Fluorescence microscopy of live cells represents a major tool in the study of trafficking events. The conventional microscope design is well adapted to image fast cellular dynamics in two dimensions, i.e., in the plane of focus. However, cells are three-dimensional objects and intracellular trafficking pathways are typically not constrained to one focal plane. If the dynamics are not constrained to one focal plane, the conventional single plane microscopy technology is inadequate for detailed studies of fast intracellular dynamics in three dimensions. Classical approaches based on changing the focal plane are often not effective in such situations since the focusing devices are relatively slow in comparison to many of the intracellular dynamics. In addition, the focal plane may frequently be at the wrong place at the wrong time, thereby missing important aspects of the dynamic events. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Multifocal plane microscopy」の詳細全文を読む スポンサード リンク
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